@article {3132724,
	title = {Dual function of C/D box small nucleolar RNAs in rRNA modification and alternative pre-mRNA splicing},
	journal = {Proc Natl Acad Sci U S A},
	volume = {113},
	number = {12},
	year = {2016},
	note = {1091-6490Falaleeva, MarinaPages, AmadisMatuszek, ZanetaHidmi, SanaAgranat-Tamir, LilyKorotkov, KonstantinNevo, YuvalEyras, EduardoOrcid: 0000-0003-0793-6218Sperling, RuthStamm, Stefan01GM079549/GM/NIGMS NIH HHS/United StatesP20 GM103486/GM/NIGMS NIH HHS/United StatesS10RR029127/RR/NCRR NIH HHS/United StatesP20GM103486-09/GM/NIGMS NIH HHS/United StatesR01 GM083187/GM/NIGMS NIH HHS/United StatesS10 RR029127/RR/NCRR NIH HHS/United States01GM083187/GM/NIGMS NIH HHS/United StatesR01 GM079549/GM/NIGMS NIH HHS/United StatesJournal ArticleResearch Support, N.I.H., ExtramuralResearch Support, Non-U.S. Gov{\textquoteright}tProc Natl Acad Sci U S A. 2016 Mar 22;113(12):E1625-34. doi: 10.1073/pnas.1519292113. Epub 2016 Mar 8.},
	month = {Mar 22},
	pages = {E1625-34},
	edition = {2016/03/10},
	abstract = {C/D box small nucleolar RNAs (SNORDs) are small noncoding RNAs, and their best-understood function is to target the methyltransferase fibrillarin to rRNA (for example, SNORD27 performs 2{\textquoteright}-O-methylation of A27 in 18S rRNA). Unexpectedly, we found a subset of SNORDs, including SNORD27, in soluble nuclear extract made under native conditions, where fibrillarin was not detected, indicating that a fraction of the SNORD27 RNA likely forms a protein complex different from canonical snoRNAs found in the insoluble nuclear fraction. As part of this previously unidentified complex,SNORD27 regulates the alternative splicing of the transcription factor E2F7p re-mRNA through direct RNA-RNA interaction without methylating the RNA, likely by competing with U1 small nuclear ribonucleoprotein (snRNP). Furthermore, knockdown of SNORD27 activates previously "silent" exons in several other genes through base complementarity across the entire SNORD27 sequence, not just the antisense boxes. Thus, some SNORDs likely function in both rRNA and pre-mRNA processing, which increases the repertoire of splicing regulators and links both processes.},
	keywords = {*Alternative Splicing, Alternative Splicing, Base Pairing, Base Sequence, Cell Cycle, Cell Division, Cell Fractionation/methods, Cell Nucleus/chemistry, Chromosomal Proteins, Non-Histone/analysis, E2F7 Transcription Factor/genetics, Exons/genetics, Gene Knockdown Techniques, Gene regulation, HeLa Cells, Humans, Methylation, Molecular Sequence Data, Oligonucleotides, Antisense/genetics, Organelle Biogenesis, pre-mRNA processing, Ribonucleoprotein, U1 Small Nuclear/metabolism, Ribosomes/metabolism, RNA Precursors/*metabolism, RNA Processing, Post-Transcriptional/*physiology, RNA, Ribosomal/*metabolism, RNA, Small Nucleolar/*physiology, snoRNAs, Solubility, Spliceosomes/metabolism},
	isbn = {0027-8424 (Print)0027-8424},
	author = {Falaleeva, M. and Pages, A. and Matuszek, Z. and Hidmi, S. and Agranat-Tamir, L. and Korotkov, K. and Nevo, Y. and Eyras, E. and Sperling, R. and Stamm, S.}
}
