@article {3132767,
	title = {Factors involved in early polarization of the anterior-posterior axis in the milkweed bug Oncopeltus fasciatus},
	journal = {Genesis},
	volume = {55},
	number = {5},
	year = {2017},
	note = {Ginzburg, NetaCohen, MiraChipman, Ariel DengResearch Support, Non-U.S. Gov{\textquoteright}tGenesis. 2017 May;55(5). doi: 10.1002/dvg.23027. Epub 2017 Apr 22.},
	month = {May},
	edition = {2017/04/23},
	abstract = {The axes of insect embryos are defined early in the blastoderm stage. Genes involved in this polarization are well known in Drosophila, but less so in other insects, such as the milkweed bug Oncopeltus fasciatus. Using quantitative PCR, we looked at differential expression of several candidate genes for early anterior-posterior patterning and found that none of them are expressed asymmetrically in the early blastoderm. We then used an RNA-Seq approach to identify novel candidate genes that might be involved in early polarization in Oncopeltus. We focused on transcription factors (TFs) as these are likely to be central players in developmental processes. Using both homology and domain based identification approaches, we were unable to find any TF encoding transcripts that are expressed asymmetrically along the anterior-posterior axis at early stages. Using a GO-term analysis of all asymmetrically expressed mRNAs, we found an enrichment of genes relating to mitochondrial function in the posterior at the earliest studied time-point. We also found a gradual enrichment of transcription related activities, giving us a putative time frame for the maternal to zygotic transition. Our dataset provides us with a list of new candidate genes in early development, which can be followed up experimentally.},
	keywords = {*Body Patterning, *early patterning, *mitochondria, *qPCR, *RNAseq, *Transcription Factor, Animals, Female, Gene Expression Regulation, Developmental, Hemiptera/embryology/*genetics, Insect Proteins/*genetics/metabolism, Male, Transcription Factors/*genetics/metabolism, Zygote/metabolism},
	isbn = {1526-968X (Electronic)1526-954X (Linking)},
	author = {Ginzburg, N. and Cohen, M. and Chipman, A. D.}
}
