@article {3132795,
	title = {The hippocampal transcriptomic signature of stress resilience in mice with microglial fractalkine receptor (CX3CR1) deficiency},
	journal = {Brain Behav Immun},
	volume = {61},
	year = {2017},
	note = {Rimmerman, NetaSchottlender, NofarReshef, RonenDan-Goor, NadavYirmiya, RazengNetherlandsBrain Behav Immun. 2017 Mar;61:184-196. doi: 10.1016/j.bbi.2016.11.023. Epub 2016 Nov 23.},
	month = {Mar},
	pages = {184-196},
	edition = {2016/11/29},
	abstract = {Clinical studies suggest that key genetic factors involved in stress resilience are related to the innate immune system. In the brain, this system includes microglia cells, which play a major role in stress responsiveness. Consistently, mice with deletion of the CX3CR1 gene (CX3CR1(-/-) mice), which in the brain is expressed exclusively by microglia, exhibit resilience to chronic stress. Here, we compared the emotional, cognitive, neurogenic and microglial responses to chronic unpredictable stress (CUS) between CX3CR1(-/-) and wild type (WT) mice. This was followed by hippocampal whole transcriptome (RNA-seq) analysis. We found that following CUS exposure, WT mice displayed reduced sucrose preference, impaired novel object recognition memory, and reduced neurogenesis, whereas CX3CR1(-/-) mice were completely resistant to these effects of CUS. CX3CR1(-/-) mice were also resilient to the memory-suppressive effect of a short period of unpredictable stress. Microglial somas were larger in CX3CR1(-/-) than in WT, but in both genotypes CUS induced a similar decline in hippocampal microglial density and processes length. RNA sequencing and pathway analysis revealed basal strain differences, particularly reduced expression of interferon (IFN)-regulated and MHC class I gene transcripts in CX3CR1(-/-) mice. Furthermore, while CUS exposure similarly altered neuronal gene transcripts (e.g. Arc, Npas4) in both strains, transcripts downstream of hippocampal estrogen receptor signaling (particularly Igf2 and Igfbp2) were altered only in CX3CR1(-/-) mice. These findings indicate that emotional and cognitive stress resilience involves CX3CR1-dependent basal and stress-induced alterations in hippocampal transcription, implicating inhibition of CX3CR1 signaling as a novel approach for promoting stress resilience.},
	keywords = {*Transcriptome, Animals, Chronic unpredictable stress, CX3C Chemokine Receptor 1/*genetics/metabolism, Cx3cr1, depression, Hippocampus, Hippocampus/*metabolism, Igf2, Interferon, Male, Memory/physiology, Mhc-i, Mice, Mice, Knockout, microglia, Microglia/*metabolism, Neurogenesis, Neurogenesis/physiology, Neurons/metabolism, Resilience, Psychological, RNA sequencing, Signal Transduction, stress, Stress, Psychological/genetics/*metabolism},
	isbn = {1090-2139 (Electronic)0889-1591 (Linking)},
	author = {Rimmerman, N. and Schottlender, N. and Reshef, R. and Dan-Goor, N. and Yirmiya, R.}
}
