Date Published:

Jul

Abstract:

The molecular program underlying infrequent replication of pancreatic β-cells remains largely inaccessible. Using transgenic mice expressing green fluorescent protein in cycling cells, we sorted live, replicating β-cells and determined their transcriptome. Replicating β-cells upregulate hundreds of proliferation-related genes, along with many novel putative cell cycle components. Strikingly, genes involved in β-cell functions, namely, glucose sensing and insulin secretion, were repressed. Further studies using single-molecule RNA in situ hybridization revealed that in fact, replicating β-cells double the amount of RNA for most genes, but this upregulation excludes genes involved in β-cell function. These data suggest that the quiescence-proliferation transition involves global amplification of gene expression, except for a subset of tissue-specific genes, which are "left behind" and whose relative mRNA amount decreases. Our work provides a unique resource for the study of replicating β-cells in vivo.

Notes:

1939-327xKlochendler, AgnesCaspi, InbalCorem, NoaMoran, MayaFriedlich, OrielElgavish, SharonaNevo, YuvalHelman, AharonGlaser, BenjaminEden, AmirItzkovitz, ShalevDor, YuvalJournal ArticleResearch Support, N.I.H., ExtramuralResearch Support, Non-U.S. Gov'tDiabetes. 2016 Jul;65(7):2081-93. doi: 10.2337/db16-0003. Epub 2016 Mar 18.